第1章 單細胞操作與體外實驗技術創建 1.從油菜(Brassica napus L.CV.Topas)裂外壁小孢子培育出胚狀體與小植株 2.Cotyledon.derived Diploid and Haploid Protoplast Culture and Diploid Plant Regeneration in Brassica napus CV.Topas 3.Single-Embryo RT-PCR Assay for the Studies of Gene Expression Dynamics During Embryogenesis in Arabidopsis Thaliana 4.Embryo Sac Isolation inArabidopsis Thaliana：A Simple and Efficient Technique for Structure Analysis and Mutant Selection 5.Regeneration of Fertile Plants from Isolated Tobacco Zygotes by／n Vitro Culture 6.Single-Embryo RT-PCR：Application for Gene Expression Dynamics and Some Technical Aspects 7.Probing Lectin Binding Sites on Isolated Viable Generative and Sperm Cells 0f Tobacco 8.Single Microspore Reverse Transcription-polymerase Chain Reaction Dynamic Change of Gene Expression During Microsporogenesis 9.An Improved Method for the Preservation and Location of Single Cell During Embedding Procedure for Transmission Electron Microscopy 10.Improved and High Throughput Quantitative Meurements of Weak GFP Expression in Transgenic Plant Materials 11.The Isolation of Early Nuclear Endosperm of Ovyza Sativa to Facilitate Gene Expression Analysis and Screening Imprinted Genes 12.An Efficient Antipodal Cell Isolation Method for Transcriptome Analysis and Screening of Cell Type-speciflc Genes in Arabidopsis Thaliana 第2章 配子與配子體發育研究 1.宜昌泡桐雌雄配子體發育及其生殖過程的研究 2.Mosaicism in the Organization of Con A Binding Sites on the Membrane Surface of Female Cells ofNicotiana Tabacum 3.The Stereotyped Positioning of the Generative Cell Associated with Vacuole Dynamics Is Not Required for Male Gametogenesis in Rice Pollen 4.Quantum Dot.Mediated Detection of 1一Aminobutyric-Acid-Binding Sites on the Surface of Living PoUen Protoplasm in Tobacco 5.Exogenous GABA Affects Ponen Tube Growth via Modulating Putative Ca2+Permeable Membrane Channels and Is Coupled with Negative Regulation on Glutamate Decarboxylase 6.NtGNLl is Involved in Embryonic Cell Division Paeming，Root Elongation and Pollen Tube Growth in Tobacco 7.The Arabidopsis Exine Formation Defect(EFD)Gene is Required for Primexine Patterning and is Critical for Pollen Fertility 8.NtProRPl，a Novel Proline.rich Protein，Is an Osmotic Stress Responsive Factor and Specifically Functions in Pollen Tube Growth and Early Embryogenesis in Nicotiana Tabacum 9.OsFIE2 Plays an Essential Role in the Regulation of Rice Vegetative and Reproductive Development 10.Mitochondrial GCDl Dysfunction Reveals Reciprocal Cell-to-cell Signaling During the Maturation ofArabidopsis Female Gametes 11.Autophagy-mediated Compartmental Cytoplasmic Deletion Is Essential for Tobacco Pollen Germination and Male Fertih'ty 12.H3K27 Methylation Regulates the Fate of Two Cell Lineages in Male Gametophytes 第3章 植物體外受精與受精機制研究 1.分離煙草胚囊的新方法及誘導卵細胞與助細胞原生質體的原位融合 2.用聚乙二醇誘導選定的成對原生質體間的融合 3.煙草雌性細胞原生質體的融合實驗 4.煙草中央細胞離體受精過程中雌雄核融合生活動態的記錄 5.In Vitro Double Fertilization in Nicotiana Tabacum(L.)：Fusion Behavior and Gamete Interaction Traced by Video-enhanced Microscopy 6.In Vitro Double Fertilization in Nicotiana Tabacum(L.)：Polygamy Compared with Selected Single Pair Somatic Protoplast and Chloroplast Fusions 7.In Vitro Double Fertilization in Nicotiana Tabacum(L.)：the Role of Cell Volume in Cell Fusion 8.In Vitro Fertilization in Maize and Tobacco：the Fusion Patterns Du 12.Fertilized Egg Cells Secrete Endopeptidases to Avoid Polytubey 13.In Vitro Fertilization of Angiosperms--10 Year Effort in China 第4章 父母親本基因在受精后發育中的作用 1.Differential Gene Expression in Egg CeUs and Zygotes Suggests that the Transcriptome is Restructed Before the First Zygotic Division in Tobacco 2.The Maternal-to-zygotic Transition Occurs Before the First Zygotic Division and de novo Transcripts are Ukely Required After Fertilization for Triggering Embryogenesis in Tobacco 3.Expressed Sequence-tag Analysis of Tobacco Sperm CeHs Reveals a Unique Transcriptional Profile and the Specific Distribution Pattern of Selected Paternal Transcripts 4.Two.step Maternal-to-zygotic Transition with Two-phase Parental Genome Contributions 5.Equal Parental Contribution to the Transcriptome Is Not Equal Control of Embryogenesis 6.The Parental Contributions to Early Plant Embryogenesis and the Concept of Maternal-to.zygotic Transition in Plants 第5章 早期胚胎髮生研究 1.多胚水稻胚胎學研究——反足細胞的發育及特點 2.Establishment of a Simple and Efficient System for Somatic Embryo Induction via Ovule Culture in Arabidopsis Thaliana 3.Tobacco Zygotic Embryogenesis in Vitro：the Original Cell Wall of the Zygote Is Essential for the Maintenance of Cell Polarity，the Apical-basal Axis and Typical Suspensor Formation 4.Exine-dehisced Microspores：a Novel Model System for Studying Embryogenesis 5.Polar Protein Transport Between Apical and Basal Cells During Tobacco Early Embryogenesis 6.Exine Dehiscing Induces Rape Microspore Polarity.Wmch Results in Different Cell Fate and Fixes the Apical Basal Axis of Embryo Genesis，but Uncouples Asymmetric Cell Division 7.A Bipartite Molecular Module Controls Cell Death Activation in the Basal Cell Lineage of Plant Embryos 8.NtDRP Is Necessary for Accurate Zygotic Division Orientation and Differentiation of Basal Cell Lineage Toward Suspensor Formation 9.Initiation of Programed Cell Death in Embryo Suspensor Is Maternal-predominantly Regulated in a Tobacco Hybrid 10.Direct Evidence That Suspensor Cells Have Embryogenic Potential That Is Suppressed by the Embryo Proper During Normal Embryogenesis 11.OGCD1 Is Essential for Rice Fertility and Required for Embryo Dorsal-ventral Pattern Formation and Endosperm Development 12.r11he Autonomous Cell Fate Specification of Basal Cell Lineage：the Initial Round of Cell Fate Specification Occurs at the Two-celled Proembryo Stage 13.A VPE-like Protease NtTPE8 Exclusively Expresses in the Integumentary Tapetum and Involves in Seed Development 14.Maternal Control of Suspensor Programmed Cell Death via Gibberellin Signaling 15.AtMIFl Increases Seed Oil Content by Attenuating GL2 Inhibition 16.Endosperm Development Is an Autonomously Programmed Process Independent of Embryogenesis 後記
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